Particulate matter 2.5 induces autophagy via inhibition of the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin kinase signaling pathway in human bronchial epithelial cells
Particulate matter 2.5 (PM2.5) is a significant risk factor for asthma. A recent study revealed that autophagy was associated with asthma pathogenesis. However, the specific mechanisms underlying PM2.5induced autophagy in asthma have remained elusive. In the present study, PM2.5induced
autophagy was evaluated in Beas2B human bronchial epithelial cells and the potential molecular mechanisms were investigated. Using electron microscopy, immunofluorescence staining and immunoblot studies, it was confirmed that PM2.5 induced autophagy in Beas2B cells as a result of PM2.5mediated
inhibition of the phosphatidylinositol 3kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway in Beas2B cells. LY294002, a PI3K inhibitor, reduced the accumulation of microtubuleassociated protein 1 light chain 3 II and attenuated the effect of PM2.5. Phosphorylated
(p)p38, pextracellular signalregulated kinase and pcJun Nterminal kinase were dephosphorylated following exposure to PM2.5. The roles of p53, reactive oxygen species scavenger tetramethylthiourea and autophagy inhibitor 3methyladenine in PM2.5induced autophagy in Beas2B cells were also
investigated. The results suggested that the PI3K/Akt/mTOR signaling pathway may be a key contributor to PM2.5induced autophagy in Beas2B cells. The results of the present study therefore provided an a insight into potential future clinical applications targeting these signaling pathways,
for the prevention and/or treatment of PM2.5induced lung diseases.
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Document Type: Research Article
Clinical Research Center, Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China
Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China
Department of Medicine, Division of Pulmonary and Critical Care Medicine, Northwestern University Feinberg School of Medicine and Jesse Brown VA Medical Center, Chicago, IL 60611, USA
Department of Immunology and Tumor Research Institute, The First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shanxi 710061, P.R. China
Publication date: August 1, 2015
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Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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