The present study aimed to investigate changes at the transcript level that are associated with spontaneous astrocytoma progression, and further, to discover novel targets for glioma diagnosis and therapy. GSE4290 microarray data downloaded from Gene Expression Omnibus were used to
identify the differentially expressed genes (DGEs) by significant analysis of microarray (SAM). The Short Time Series Expression Miner (STEM) method was then applied to class these DEGs based on their degrees of differentiation in the process of tumor progression. Finally, EnrichNet was used
to perform the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis based on a proteinprotein interaction (PPI) network. A total of 4,506 DEGs were detected, and the number of DEGs was the highest in grade IV cells (2,580 DEGs). These DEGs were classified into nine
clusters by the STEM method. In total, 11 KEGG pathways with XDscores larger than the threshold (0.96) were obtained. The DEGs enriched in pathways 1 (extracellular matrixreceptor interaction), 3 (phagosome) and 6 (type I diabetes mellitus) mainly belonged to cluster 5.
Pathway 2 (longterm potentiation), 4 (Vibrio cholerae infection) and 5 (epithelial cell signaling in Helicobacter pylori infection) was involved with DEGs that belonged to different clusters. Significant changes in gene expression occurred during glioma progression. Pathways 1, 3
and 6 may be important for the deterioration of glioma into glioblastoma, and pathways 2, 4 and 5 may have a role at each stage during glioma progression. The associated DEGs, including SV2, NMDAR and mGluRs, may be suitable as biomarkers or therapeutic targets for gliomas.
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Document Type: Research Article
Department of Emergency Medicine, ChinaJapan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
Department of Neurosurgery, ChinaJapan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
Department of Ophthalmology, ChinaJapan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
Department of Opthalmology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Department of Neurology, Jilin Oilfield General Hospital, Songyuan 131200, P.R. China
Publication date: August 1, 2015
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Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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