Neuroglobin (NGB) is a recently identified protein, which is localized in the neurons and retinal cells of the central and peripheral nervous systems in vertebrates. It is hypothesized to function as a scavenger for reactive oxygen species, or as a stressresponsive sensor for signal
transduction in hypoxicischemic brain insults. However, the mechanism underlying the physiological function of this protein remains to be elucidated. In the present study, the profiling of changes in the serum redox index of morphological features of the hippocampus and cortex, and of the
expression of NGB and hypoxiainducible factor1α (HIF1α), are described in a rat middle cerebral artery occlusion (MCAO) model. The necrotic zone of the rat neural tissues increased in size with increasing reperfusion time, and different brain slices exhibited necrosis in different
regions. The number of NGBpositive hippocampal and cortical cells, as well as NGB and HIF1α transcript and protein levels in the ischemic cortex, increased with increasing reperfusion time. NGB and HIF1α mRNA and protein levels peaked in the group that received reperfusion at 32 h
after MCAO. These findings indicated that HIF1α may be involved in ischemic pathology in an MCAO model and that NGB expression may be upregulated. Serum superoxide dismutase (SOD) activity decreased and serum malondialdehyde (MDA) levels increased with increasing reperfusion time, indicating
that the redox potential increased following MCAO. Serum SOD and MDA measurements may, therefore, be useful as biomarkers for the early detection of ischemic injury in a clinical setting.
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Document Type: Research Article
Department of Neurosurgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China
Department of Health Medicine, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China
Department of Pediatrics, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China
National Center for Scientific Data Sharing for Population and Health, Beijing 100730, P.R. China
Medical Neurobiology of State Key Laboratory, Fudan University, Shanghai 200032, P.R. China
Publication date: August 1, 2015
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Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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