Establishment of an interleukin1βinduced inflammationactivated endothelial cellsmooth muscle cellmononuclear cell coculture model and evaluation of the antiinflammatory effects of tanshinone IIA on atherosclerosis
Increasing evidence supports the hypothesis that inflammatory reactions serves an important function in the formation, progression and plaque rupture of atherosclerosis. Interleukin (IL)1 primarily induces inflammation and is closely associated with the inflammatory environment and the formation of atherosclerosis. The present study aimed to establish an in vitro model for the evaluation of drug efficacy in the intervention of atherosclerosis from the inflammatory perspective, and to observe the antiinflammatory effects of tanshinone IIA and andrographolide on atherosclerosis. The IL1βinduced inflammationactivated endothelial cell (EC)smooth muscle cell (SMC)mononuclear cell (MC) coculture model was established, based on the changes in a series of atherosclerosisassociated inflammatory markers secreted by ECs and SMCs. The expression of connexin in ECs, adhesion of MCs and changes in inflammatory signalling molecules were selected as evaluation indices for the inflammatory microenvironment of atherosclerosis. The use of this model revealed that tanshinone IIA exhibited significant efficacy against atherosclerosis and its inflammatory reactions. Inflammatory reactions were regarded as the primary mechanism underlying atherosclerosis. The established model simulated a series of relevant changes in the arterial wall under the inflammatory cytokines with oxidized lowdensity lipoprotein during the atherosclerotic process. The present study presented a reliable method for the identification of drugs with potential antiinflammatory activity in atherosclerosis, for investigating the mechanisms of action, considering the improvement of the inflammatory state and the increase in plaque stability observed.
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Document Type: Research Article
Affiliations: Department of Pharmacokinetics of Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, P.R. China
Publication date: August 1, 2015
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