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Effects of the nineinlike protein centrosomal protein on breast cancer cell invasion and migration

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To investigate the effects of the centrosomal protein, nineinlike protein (Nlp), on the proliferation, invasion and metastasis of MCF7 breast cancer cells, the present study established green fluorescent protein (GFP)containing MCF7 plasmids with steady and overexpression of Nlp (MCG7GFPN1p) and blank plasmids (MCF7GFP) using lentiviral transfection technology in MCF7 the breast cancer cell line. The expression of Nlp was determined by reverse transcriptionquantitative polymerase chain reaction and western blott analysis. Differences in levels of proliferation, invasion and metastasis between the MCF7GFPNlp group and MCFGFP group were compared using MTT, plate colony formation and Transwell migration assays. The cell growth was more rapid and the colony forming rate was markedly increased in the MCF7GFPNlp group (P<0.05) compared with the MCF7GFP group. The number of cells in the MCFGFPNlp and MCF7GFP groups transferred across membranes were 878±18.22 and 398±8.02, respectively, in the migration assay. The invasive capacity was significantly increased in the MCF7GFPNlp group (P<0.05) compared with the MCF7GFP group. The western blotting results demonstrated high expression levels of CXC chemokine receptor type 4 in the MCF7GFPNlp group. The increased expression of Nlp was associated with an increase in MCF7 cell proliferation, invasion and metastasis, which indicated that Nlp promoted breast tumorigenesis and may be used as a potent biological index to predict breast cancer metastasis and develop therapeutic regimes.
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Document Type: Research Article

Affiliations: 1: Department of II, Shandong Breast Center of Prevention and Treatment, Shandong Cancer Hospital, Jinan, Shandong 250117, P.R. China 2: Clinical Laboratory, Haiyang People's Hospital, Yantai, Shandong 265100, P.R. China

Publication date: August 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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