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Apigenin inhibits the self-renewal capacity of human ovarian cancer SKOV3derived sphere-forming cells

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Casein kinase 2 (CK2) is a protein kinase which is frequently activated in cancer. The Hedgehog (Hh) signaling pathway is involved in the stimulation of cancer stem cell growth. Its aberrant activation has been validated in several types of cancer, including ovarian cancer. In the present study, the sphereforming cells (SFCs) of the human ovarian cancer SKOV3 cell line were observed to have selfrenewal capacity, indicating the possession of ovarian cancer stemlike cell properties. SKOV3derived SFCs had higher levels of CK2α and gliomaassociated oncogene¬†1¬†(Gli1) proteins compared with those of parental cells. Apigenin, a common flavonoid, significantly inhibited the selfrenewal capacity and the protein expression of CK2α and Gli1 proteins in the SKOV3derived SFCs, which occurred in a concentrationdependent manner. In addition, CK2α small interfering RNA downregulated the protein expression of CK2α and Gli1 and synergistically inhibited the selfrenewal capacity of the SKOV3derived SFCs with apigenin. However, forced overexpression of CK2α resulted in an increase in the expression of CK2α and Gli1 and attenuated the apigenininhibited selfrenewal effect in the SKOV3derived SFCs. These results suggested that apigenin inhibited the selfrenewal capacity of SKOV3derived SFCs and was involved in downregulating the expression of Gli1 by the inhibition of CK2α.
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Document Type: Research Article

Affiliations: 1: Department of Gynaecology and Obstetrics, The Maternal and Child Health Hospital of Hunan Province, Changsha, Hunan 410008, P.R. China 2: Laboratory of Medicine Engineering, Medical College, Hunan Normal University, Changsha, Hunan 410013, P.R. China

Publication date: March 1, 2015

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  • Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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