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Hypoxia upregulates Hsp90α expression via STAT5b in cancer cells

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Hsp90α is a molecular chaperone protein involved in the structural maturation of oncogenic signaling proteins. Hsp90 was recently identified as an anticancer target; various studies are ongoing to find ways for managing cancer through Hsp90α. However, this approach is limited by reported side-effects. Hypoxia is a hallmark of solid tumors, including those of breast cancer and the extent of tumor hypoxia is associated with resistance to treatment and poor prognosis. One of the major signaling pathways in cancer cells, the Jak2/STAT5b pathway, has been found to be closely correlated with hypoxia. The objective of this study was to investigate the role of Jak2/STAT5b in the regulation of Hsp90α expression so that Hsp90α targeting can be achieved indirectly by modulating the Jak2/STAT5b pathway. We examined the role of the Jak2/STAT5b pathway in the expression of Hsp90α under hypoxic conditions by immunoblotting, reporter gene assays, EMSA and RNA interference analysis. With the help of in┬ávivo models, we also analyzed the expression of Hsp90α in different parts of solid tumor tissues. We found a close association between hypoxic stress and Hsp90α expression. We also determined that STAT5b regulates the expression of Hsp90α during hypoxic stimulation. Under hypoxic conditions the expression of Hsp90α and STAT5b were proportional. siRNA analysis and nucleotide analysis showed that the promoter of Hsp90α has a STAT5b binding domain. Our work confirmed that STAT5b is one of the transcription factors that regulate Hsp90α. We, therefore, concluded that under hypoxic conditions, the Jak2/STAT5b pathway regulates Hsp90α expression and it could serve as a promising target for the treatment of solid tumors.
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Document Type: Research Article

Affiliations: 1: Department of Pathology, School of Medicine, and Institute of Biomedical Science and Technology, Konkuk University Hospital, Seoul 143-701, Republic of Korea 2: Emergency Medicine, Konkuk University Hospital, Seoul 143-701, Republic of Korea 3: Konkuk University Hospital, Seoul 143-701, Republic of Korea 4: Bio-Food and Drug Research Center, Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Republic of Korea 5: Department of Pharmacology, School of Medicine, Seoul National University, Seoul 110-799, Republic of Korea 6: Department of Internal Medicine, School of Medicine, Konkuk University Glocal Campus, Chungju 380-701, Republic of Korea 7: Department of Animal Science, College of Life Sciences, Pusan National University, Busan 609-735, Republic of Korea 8: Genomic Informatics Center, Hankyong National University, Anseong 456-749, Republic of Korea 9: Department of Applied Life Science, Konkuk University, Seoul 143-701, Republic of Korea

Publication date: January 1, 2012

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  • The International Journal of Oncology provides an international forum for the publication of the latest, cutting-edge research in the broad area of oncology and cancer treatment. The journal accepts original high quality works and reviews on all aspects of oncology research including carcinogenesis, metastasis, epidemiology, chemotherapy and viral oncology. Through fair and efficient peer review, the journal is dedicated to publishing top tier research in the field, offering authors rapid publication as well as high standards of copy-editing and production. The International Journal of Oncology is published on a monthly basis in both print and early online.
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