Prevention of PU.1-induced growth inhibition and apoptosis but not differentiation block in murine erythroleukemia cells by overexpression of CBP
PU.1 is a member of Ets family of transcription factors, some of which play critical roles in cell growth and development of hematopoietic cells in cooperation with other transcription factors and cofactors. We previously reported that overexpression of PU.1 in murine erythroleukemia
(MEL) cells results in growth inhibition, differentiation block and apoptotic cell death in conjunction with DMSO or HMBA treatment. We also showed that PU.1 interacts with the transcriptional co-activator CREB binding protein (CBP). In this study, we have investigated whether CBP is involved
in PU.1-induced growth inhibition, differentiation block and apoptosis in MEL cells. Overexpression of CBP rescued MEL cells from PU.1-induced growth inhibition and apoptosis, while it did not release the cells from PU.1-induced block of erythroid differentiation. These results suggest that
sequestration of CBP is involved in PU.1-induced growth inhibition and apoptosis but not differentiation block in MEL cells. These results also suggest that the molecular mechanisms on PU.1-induced growth inhibition and apoptosis are different from the mechanism on PU.1-induced differentiation
block in MEL cells.
Document Type: Research Article
Affiliations: Department of Cell Genetics, Sasaki Institute, Tokyo 101-0062, Japan
Publication date: 01 June 2003
- The International Journal of Oncology provides an international forum for the publication of the latest, cutting-edge research in the broad area of oncology and cancer treatment. The journal accepts original high quality works and reviews on all aspects of oncology research including carcinogenesis, metastasis, epidemiology, chemotherapy and viral oncology. Through fair and efficient peer review, the journal is dedicated to publishing top tier research in the field, offering authors rapid publication as well as high standards of copy-editing and production. The International Journal of Oncology is published on a monthly basis in both print and early online.
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