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Expression and synthesis of insulin-like growth factor (IGF)-I, -II and their receptors in human glioma cell lines

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Insulin-like growth factor (IGF)-I and -II are involved in the regulation of brain development and are thought to play a pivotal role in the proliferation of gliomas. Expression of IGF-I, IGF-II, the type I and type II IGF receptor were studied in a panel of thirty glioma cell lines by Northern blotting and PCR analysis. IGF-II mRNA expression with transcripts of 4.8 and 6.0 kb was shown only in one glioma cell line (NCE-G96) and no transcripts for IGF-I, IGF-I-R and IGF-II-R could be detected by Northern analysis in total RNA. However, PCR analysis revealed signals in 19/28 cell lines for IGF-I, 27/30 for ICE-II, 19/28 for IGF-I-R and 22/28 glioma cell lines for IGF-II-R. Additional IGF-I, IGF-II, IGF-I-R and IGF-II-R PCR products were detected which might represent alternative splicing products or variants. In addition, the secretion of IGF-I and IGF-II peptides was measured by radioimmunoassay. IGF receptor status and binding characteristics were established by Scatchard analysis. Proliferation assays showed different effects of IGFs and IGF analogues on the proliferation of these cell lines. Des-(1-3)IGF-I showed an unexpected inhibitory activity on glioma cell proliferation. This may have either been due to a direct effect of the ligand for the induction of a more differentiated state refractory to its action.
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Document Type: Research Article

Affiliations: 1: UNIV HAMBURG,HOSP EPPENDORF,LAB BRAIN TUMOUR BIOL,DEPT PEDIAT,D-20246 HAMBURG,GERMANY. UNIV HAMBURG,HOSP EPPENDORF,LAB BRAIN TUMOUR BIOL,DEPT NEUROSURG,D-20246 HAMBURG,GERMANY. UNIV CAMBRIDGE,DEPT ANAT,CAMBRIDGE CB2 1TN,ENGLAND. INST CURIE,LAB ONCOGENESE RETROVIRALE & MOL,F-91405 ORSAY,FRANCE. 2: UNIV HAMBURG,HOSP EPPENDORF,LAB BRAIN TUMOUR BIOL,DEPT PEDIAT,D-20246 HAMBURG,GERMANY. UNIV HAMBURG,HOSP EPPENDORF,LAB BRAIN TUMOUR BIOL,DEPT NEUROSURG,D-20246 HAMBURG,GERMANY. UNIV CAMBRIDGE,DEPT ANAT,CAMBRIDGE CB2 1TN,ENGLAND. INST CURIE,LAB ONCOGENESE RETROVIRALE & MOL,F-91405 ORSAY,FRANCE.

Publication date: November 1, 1996

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  • The International Journal of Oncology provides an international forum for the publication of the latest, cutting-edge research in the broad area of oncology and cancer treatment. The journal accepts original high quality works and reviews on all aspects of oncology research including carcinogenesis, metastasis, epidemiology, chemotherapy and viral oncology. Through fair and efficient peer review, the journal is dedicated to publishing top tier research in the field, offering authors rapid publication as well as high standards of copy-editing and production. The International Journal of Oncology is published on a monthly basis in both print and early online.
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