PCR DETECTION OF CLONAL TCR GAMMA-GENE REARRANGEMENTS IN A GROUP OF CUTANEOUS T-CELL LYMPHOMAS INCLUDING A CASE OF LOCALIZED PAGETOID RETICULOSIS EXPRESSING THE GAMMA-DELTA TCR
In this report, we describe the analysis of clonality performed using a PCR-based approach in a group of cutaneous T-cell lymphomas including a case of localised pagetoid reticulosis expressing the gamma delta T-cell receptor. For our PCR analysis DNA was extracted from frozen tissue of 10 cases of cutaneous T-cell lymphomas and subjected to polymerase chain reaction amplification using primers recognizing conserved sequences of the variable and joining regions of the T-cell receptor gamma chain gene. PCR products were then analyzed by high resolution non-denaturing polyacrylamide gel electrophoresis. Clonal TCR gamma gene rearrangements were detected in all the cases investigated including the case of localised pagetoid reticulosis. The PCR-based approach used in this study may provide an alternative to conventional Southern blot analysis for the analysis of cutaneous T-cell lymphomas as it is easy to perform and requires only minimal amounts of DNA. The findings in localized pagetoid reticulosis are consistent with the proliferation of monoclonal T-cells in lesional skin of this condition and provide evidence for the lymphoproliferative nature of this clinically benign disorder.
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Document Type: Research Article
Affiliations: HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DERMATOL UNIT,LONDON,ENGLAND.
Publication date: June 1, 1995
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