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EXPRESSION AND LOCALIZATION OF ACTIVIN RECEPTORS IN A HUMAN MYELOID-LEUKEMIA CELL-LINE, THP-1

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THP-1, a human myeloid leukemia cell line, which secretes activin-A after phorbol ester induced cell differentiation, was used to examine the possible expression and presence of activin receptors because activin, a member of the transforming growth factor beta (TGF-beta) superfamily, has been found to be up-regulated by phorbol ester. We have studied whether THP-1 cells transcribe mRNAs encoding for activin receptors II and IIB by the reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization techniques. To confirm that THP-1 cells produce activin-A, we also determined the presence of immunoreactivity for activin-A by immunocytochemistry. We observed that messenger RNA encoding activin beta A-subunit was also expressed in THP-1 cells. Furthermore, the identity of RT-PCR products was confirmed by DNA sequencing. These results indicate that activin-A and its receptors are co-expressed in THP-1 cells and suggest that activin-A may have autocrine functions during cell proliferation in these cells.
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Document Type: Research Article

Publication date: April 1, 1995

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  • The International Journal of Oncology provides an international forum for the publication of the latest, cutting-edge research in the broad area of oncology and cancer treatment. The journal accepts original high quality works and reviews on all aspects of oncology research including carcinogenesis, metastasis, epidemiology, chemotherapy and viral oncology. Through fair and efficient peer review, the journal is dedicated to publishing top tier research in the field, offering authors rapid publication as well as high standards of copy-editing and production. The International Journal of Oncology is published on a monthly basis in both print and early online.
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