MRS EVALUATION OF BRAIN-METABOLITES IN EXTRACTS FROM CELL-CULTURES, HUMAN TUMORS AND NORMAL TISSUE FROM BRAIN - CHOLESTERYL ESTER, CHOLINE CONTAINING COMPOUNDS AND CREATINE AS MARKERS FOR DEVELOPMENT, DIFFERENTIATION AND PATHOLOGY
A multi stage extraction procedure which gives the possibility to analyze both water soluble and lipid components stemming from the same specimen has been developed in our laboratory. Metabolites from brain cancer biopsies have been compared to metabolites in normal human brain, developing
mouse brain and primary mouse cell cultures of neurons and astrocytes from cerebral cortex and cerebellum. Extraction with perchloric acid (PCA) dissolves water.soluble components such as choline containing compounds, creatine, amino acids, carbohydrates and high energy phosphates. The water
insoluble fraction left after the PCA treatment was extracted with chloroform/methanol, 2/1 (vol/vol). C-13 and H-1 NMR spectra showed characteristic lipid resonances identified as those from long chain saturated and unsaturated fatty acids and cholesterol. Only glioblastomas contained detectable
amounts of cholesteryl ester suggesting this compound as marker for brain pathology. It was shown that cholesterol but not cholesteryl ester is present in cultures of neurons either alone or together with astrocytes. H-1 NMR spectra of PCA extracts from biopsies showed that the creatine/choline
containing compounds (Cr/Cho) ratios decreased and the N-acetylaspartate/Cho ratios decreased in glioblastomas as compared to normal brain. Cell cultures retain the Cr/Cho ratio characteristic of the developmental stage of the tissue they were prepared from.
Document Type: Research Article
Affiliations: UNIV TRONDHEIM,INST CANC RES,N-7005 TRONDHEIM,NORWAY. ROYAL DANISH SCH PHARM,DEPT BIOL SCI,PHARMABIOTECH RES CTR,DK-2100 COPENHAGEN,DENMARK. NORWEGIAN INST TECHNOL,DEPT BIOTECHNOL,N-7034 TRONDHEIM,NORWAY. AARHUS UNIV,DEPT CHEM,DK-8000 AARHUS,DENMARK.
Publication date: 01 April 1993
- The International Journal of Oncology provides an international forum for the publication of the latest, cutting-edge research in the broad area of oncology and cancer treatment. The journal accepts original high quality works and reviews on all aspects of oncology research including carcinogenesis, metastasis, epidemiology, chemotherapy and viral oncology. Through fair and efficient peer review, the journal is dedicated to publishing top tier research in the field, offering authors rapid publication as well as high standards of copy-editing and production. The International Journal of Oncology is published on a monthly basis in both print and early online.
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