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Inhibition of MEK/ERK/STAT3 signaling in oleuropein treatment inhibits myocardial ischemia/reperfusion

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Studies have shown that oleuropein has antifungal, antiinflammatory, antiviral, antioxidant, anticancer and hypoglycemic functions. TTC solution staining was used to measure myocardial infarction size. A commercial kit was used to measure lactate dehydrogenase (LDH), creatinine kinaseMB (CKMB), tumor necrosis factorα (TNFα), interleukin1β (IL1β), IL 6, superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA) and catalase levels. Western blot analysis was used to measure p53, p-MEK p-ERK and pIκBα protein expression. The present study reports that the protective effect of oleuropein also prevents against myocardial ischemia/reperfusion (myocardial I/R). The aim of this retrospective study was to evaluate this protective effect of oleuropein and the mechanisms by which myocardial I/R is prevented. Oleuropein inhibited myocardial infarction size, CKMB and LDH serum levels in a myocardial I/R rat model. Moreover, oleuropein also attenuated caspase3 activity, and p53, phosphorylated (p)mitogenactivated protein kinase kinase (MEK), pextracellular signalregulated protein kinase (ERK) and pIκBα protein expression. TNFα, IL1β, IL6 and MDA were decreased; SOD, GSH and catalase levels inhibited TNFα, IL1β, IL-6 and MDA levels, and increased SOD, GSH and catalase levels in myocardial I/R rats treated with oleuropein. Rats orally administered the MEK inhibitor PD0325901, in addition to oleuropein, exhibited inhibited myocardial infarction size, CKMB and LDH serum levels compared with rats treated with oleuropein only. Rats treated with MEK inhibitor also exhibited suppressed caspase3 activity, p53, pMEK pERK and pIκBα protein expression, TNFα, IL1β, IL6, SOD, GSH, MDA and catalase levels, and induced psignal transducer and activator of transcription 3 (STAT3) protein expression compared with rats treated with oleuropein only. Taken together, these results suggest that MEK/ERK/STAT3 signaling regulates the inhibition of myocardial I/R in rats treated with oleuropein.
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Document Type: Research Article

Affiliations: 1: Department of Anesthesiology, Harrison International Peace Hospital, Hebei Medical University, Hengshui, Hebei 053000, P.R. China 2: Department of General Medicine, Harrison International Peace Hospital, Hebei Medical University, Hengshui, Hebei 053000, P.R. China 3: Department of Internal MedicinePediatrics, The Fifth People's Hospital, Hengshui, Hebei 053000, P.R. China 4: Department of Pediatrics, Anping General Hospital, Anping, Hebei 053600, P.R. China

Publication date: January 1, 2018

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  • The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.

    The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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