The present study aimed to investigate the effect of the expression of interleukin (IL)18 and related markers on deep venous thrombosis (DVT) to examine their correlation. SpragueDawley rats of different models were established and were randomly assigned into three groups. The expression
of IL18, nuclear factor (NF)κB and von Willebrand factor (vWF) were detected in blood samples. The inferior vena cava (IVC) was ligated to establish the DVT model. Rat IL18 overexpression and inhibition vectors were constructed. The expression levels of IL18 and related markers
in the venous wall were compared between the model group and the control group using reverse transcriptionquantitative polymerase chain reaction and western blot analyses. Following the culture of human umbilical vein endothelial cells (HUVECs), IL18 was added to the cells, following
which the growth of the HUVECs, and changes in vWF and other endothelial functional markers were analyzed. The IVC model demonstrated complete thrombosis at 8 h and stable thrombosis at 24 h. At 24 h following model establishment, the expression levels of IL18, NFκB and vWF
were high in the blood samples with the occurrence and development of thrombosis (P<0.05). The weight, length and weight/length ratio of thrombi in each model group showed significant differences from those in the control group (P<0.05) with the overexpression of IL18, and the expression
levels of NFκB and vWF in venous tissues were altered with abnormal expression levels of IL18. IL18 damaged HUVECs and significantly increased viability in earlystage apoptosis, promoted the upregulation of vWF and Pselectin, and reduced tissue plasminogen activator. IL18 and the related
markers were closely associated with the occurrence and development of DVT.
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Document Type: Research Article
Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China
Publication date: January 1, 2018
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The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.
The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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