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miR133b regulates proliferation and apoptosis in highglucoseinduced human retinal endothelial cells by targeting ras homolog family member A

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The aim of the present study was to investigate the role of microRNA (miR)133b in highglucoseinduced human retinal endothelial cells (hRECs), particularly regarding its potential targeting of ras homolog family member A (RhoA). To establish the highglucoseinduced diabetic retinopathy (DR) model, hRECs were cultured in highglucose medium for 1, 2 and 3 days. An Annexin allophycocyanin (APC)/7aminoactinomycin D (7AAD) staining assay was performed to measure the apoptosis of hRECs. Next, the cells were transfected with miR133b inhibitors or mimics, and the cell proliferation and apoptosis were measured by MTT and AnnexinAPC/7AAD staining assays, respectively. In addition, reverse transcriptionquantitative polymerase chain reaction (RTqPCR), western blotting and immunocytochemistry were used to detect the expression levels of RhoA, Rhoassociated protein kinase 1 (ROCK1), LIM domain kinase 1 (LIMK), myosin light chain (MLC) and phosphorylated (p)MLC. It was observed that highglucose or miR133b inhibitor treatment attenuated the apoptosis of hRECs, and upregulated the mRNA and protein expression levels of RhoA, ROCK1 and LIMK, as well as the pMLC protein level, in the hRECs. However, miR133b overexpression inhibited the cell proliferation, promoted apoptosis, and downregulated the mRNA and protein levels of RhoA, ROCK1 and LIMK, as well as pMLC protein, in highglucoseinduced hRECs. In conclusion, overexpression of miR133b inhibited the proliferation and promoted apoptosis in a DR cell model by downregulating RhoA expression.
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Document Type: Research Article

Affiliations: 1: Department of Ophthalmology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China 2: Department of Ophthalmology, Wuxi Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Wuxi, Jiangsu 214071, P.R. China 3: Department of Ophthalmology, Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214023, P.R. China

Publication date: January 1, 2018

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  • The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.

    The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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