Anthocyanins inhibit high glucose-induced renal tubular cell apoptosis caused by oxidative stress in db/db mice
Oxidative stress is an important contributory factor resulting the development of kidney injury in patients with diabetes. Numerous in vitro and in vivo studies have suggested that anthocyanins, natural phenols commonly existing in numerous fruits and vegetables, exhibit important
antioxidative, antiinflammatory and antihyperlipidemic effects; however, their effects and underlying mechanisms on diabetic nephropathy (DN) have not yet been fully determined. In the present study, the regulation of apoptosis metabolism and antioxidative effects exhibited by anthocyanins
[grape seed procyanidin (GSPE) and cyanidin3Oβglucoside chloride (C3G)] were investigated, and the molecular mechanism underlying this process was investigated in vivo and in vitro. GSPE administration was revealed to suppress renal cell apoptosis, as well as suppress the expression
of Bcl2 in diabetic mouse kidneys. Furthermore, GSPE administration was demonstrated to suppress the expression of thioredoxin interacting protein (TXNIP), in addition to enhancing p38 mitogenactivation protein kinase (MAPK) and extracellular signalregulated kinase 1/2 (ERK1/2) oxidase
activity in diabetic mouse kidneys. In vitro experiments using HK2 cells revealed that C3G suppressed the generation of HGmediated reactive oxygen species, cellular apoptosis, the expression of cleaved caspase3 and the Bax/Bcl2 ratio; and enhanced the expression of cytochrome c released
from mitochondria. Furthermore, treatment with C3G was revealed to suppress the expression of TXNIP, in addition to the phosphorylation of p38 MAPK and ERK1/2 oxidase activity in HK2 cells under HG conditions. In addition, treatment with C3G was revealed to attenuate the HGinduced suppression
of the biological activity of thioredoxin, and to enhance the expression of thioredoxin 2 in HK2 cells under HG conditions. In conclusion, the present study demonstrated that anthocyanins may exhibit protective effects against HGinduced renal injury in DN via antioxidant activity.
Document Type: Research Article
Affiliations: 1: Department of Pathology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China 2: Department of Gastrointestinal Surgery Hernia and Abdominal Wall Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China 3: School of Public Health, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China
Publication date: 01 January 2018
- The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.
The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases. - Editorial Board
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