Identification of the biologically active constituents of Camellia japonica leaf and anti-hyperuricemic effect in vitro and in vivo
Camellia japonica L. is a plant of which the seeds are used as a folk medicine, and it is native to South Korea, Japan and China. In previous study, triterpenes, flavonoids, tannins and fatty acids which have antiviral, antioxidant and anti inflammatory activity were reported from
C. japonica leaf and flower. In Korea, the seed from this plant is used as a traditional medicine and in folk remedies for the treatment of bleeding and inflammation. However, the major issue associated with the use of the seed as a medicinal and/or functional food ingredient is its application
to the pharmaceutical and food industry. First, the productivity of seed extract is very much less than that of the leaf. Second, the beneficial usage of the seed extract as an alternative medicine and functional source is not yet clear. Thus, in this study, we focused on another part of the
plant, the leaf, and found that the extract of Camellia japonica leaf has a high concentration of vitamin E, rutin and other biologically active compounds related to hyperuricemia. We aimed to investigate the biological activities, namely the antioxidant activities, xanthine oxidase (XO)
inhibitory activity and antihyperuricemic effects of extract from C. japonica leaf and the phytochemicals contained therein. Ethanol extracts of C. japonica leaf (ECJL) were prepared, and the extract was used with respect to antioxidant activities, total phenolic contents and
XO inhibitory activity. The in vivo XO inhibitory activity and antihyperuricemic effects of the extract were evaluated in mice with potassium oxonateinduced hyperuricemia. To clarify the marker compounds that are responsible for the antihyperuricemic effects, several key constituents
were identified using gas chromatographymass spectrometry (GCMS) and and liquid chromatography-mass spectrometry (LC-MS). ECJL was found to have strong antioxidant activities, and in vitro XO inhibitory activity. The results of the in vivo experiments using mice demonstrated
that ECJL at the doses of 100 and 300 mg/kg inhibited hepatic XO activity and significantly attenuated hyperuricemia. To the best of our knowledge, the present study is the first report on the XO inhibitory and anti-hyperuricemic effects of ECJL, which can be therapeutically
applied in the treatment of hyperuricemia and gout.
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Document Type: Research Article
College of Pharmacy, Pusan National University, Geumjeong, Busan 46241, Republic of Korea
Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 58245, Republic of Korea
Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan, Jeonnam 58554, Republic of Korea
Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea
Department of Environmental Engineering, Mokpo National University, Muan, Jeonnam 58554, Republic of Korea
Jeollanamdo Wando Arboretum, Wando, Jeonnam 59105, Republic of Korea
Jeonnam Bioindustry Foundation, Institute of Natural Resources Research, Jangheung, Jeonnam 59338, Republic of Korea
Division of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea
Department of Landscape Architecture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea
Department of Physical Therapy, College of Health and Welfare, Dongshin University, Naju, Jeonnam 58245, Republic of Korea
January 1, 2017
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