Skip to main content
padlock icon - secure page this page is secure

Antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of Nrf-2mediated HO-1 expression in V79-4 Chinese hamster lung fibroblasts

Buy Article:

$42.00 + tax (Refund Policy)

Natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. The present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the Moraceae family. Superoxide dismutase (SOD)like activity and 2,2'azinobis(3ethylbenzothiazoline6sulfonic acid) (ABTS•+) radical scavenging activity were determined. We also investigated the cytoprotective effects of morin against hydrogen peroxide (H2O2)induced DNA damage and apoptosis in V794 Chinese hamster lung fibroblasts. Our results demonstrated that morin had strong scavenging effects against ABTS•+ radicals with enhanced SOD activity, which varied in a dose-dependent manner. Morin was found to reduce H2O2induced intracellular reactive oxygen species generation and nuclear DNA damage, and it recovered cell viability damaged by H2O2 via inhibition of mitochondrial dysfunctionmediated apoptosis. Notably, the treatment of V794 cells with morin markedly enhanced the expression of heme oxygenase1 (HO1) but not quinone oxidoreductase-1, which was associated with the increased expression and phosphorylation of nuclear factor-erythroid¬†2-related factor¬†2 (Nrf2) and the downregulation of Kelchlike ECHassociated protein¬†1 expression. Based on our findings, we conclude that morin effectively ameliorated oxidative stressinduced DNA damage through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: AntiAging Research Center, Dongeui University, Busan 614714, Republic of Korea 2: Department of Parasitology and Genetics, Kosin University, College of Medicine, Busan 602702, Republic of Korea 3: Natural Products Research Team, Marine Biodiversity Institute of Korea, Seocheon 325902, Republic of Korea 4: Department of Food Science and Biotechnology, College of Engineering, Kyungsung University, Busan 608736, Republic of Korea 5: Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690756, Republic of Korea 6: Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 614052, Republic of Korea 7: Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dongeui University, Busan 614714, Republic of Korea 8: School of Food Science and Technology, ChungAng University, Anseong 456756, Republic of Korea 9: Department of Dental Hygiene, College of Health Sciences, Youngsan University, Yangsan 626790, Republic of Korea 10: Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 501759, Republic of Korea 11: Department of Urology, Chungbuk National University, College of Medicine and Institute for Tumor Research, Cheongju 362763, Republic of Korea

Publication date: January 1, 2017

More about this publication?
  • The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.

    The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Information for Advertisers
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more