Lactoferricin B (LfcinB), a peptide of bovine lactoferrin (LfB), exhibits multiple biological functions, including antimicrobial, antiviral, antioxidant and immunomodulatory activities. However, the role of LfcinB-related peptides in melanogenesis remains unclear. In this
study, a set of five LfcinB-related peptides was examined. We found that LfB1734, an 18-mer LfcinB-derived peptide, increased melanogenesis in B16F10 melanoma cells without significantly affecting cell viability. LfB1734 increased in vitro tyrosinase activity and melanin content in a
dose-dependent manner. The results of RT-qPCR and western blot analyses showed that LfB1734 increased the mRNA and protein expression of tyrosinase and tyrosinase-related protein 1 (Trp1). Moreover, LfB1734 inhibited the phosphorylation of MAPK/Erk, but not p38 and Akt, and constitutively
active MEK was able to reverse the LfB17-34-enhanced pigmentation, melanin content, and tyrosinase activity, suggesting a role of Erk signaling in the process of LfB1734-mediated pigmentation. Taken together, these results suggest that LfB1734 induces melanogenesis in B16F10 cells primarily
through increased tyrosinase expression and activity and that LfB1734 could be further developed for the treatment of hypopigmentation disorders.
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Document Type: Research Article
Renorigin Innovation Institute, Taipei 11560, Taiwan, R.O.C.
Graduate Institute of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei 100, Taiwan, R.O.C.
Publication date: January 1, 2017
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The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.
The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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