Hyposalivation is an intractable sideeffect of radiotherapy for head and neck cancer. It is caused by the irreversible loss of acinar cells and decreased saliva secretion. However, this situation severely compromises the quality of life of affected patients. Currently, there is no effective
treatment for this condition. In the present study, we developed a novel approach to regenerate the function of the irradiationdamaged salivary glands using human adipose tissuederived stem cell (hADSC) intraglandular transplantation. ZsGreenlabeled hADSCs were adoptively transferred
into SpragueDawley (SD) rat submandibular glands immediately following exposure to 18 Gy irradiation. A higher salivary flow rate (SFR) was observed in the hADSCtreated group. Tissue improvement, including angiogenesis, antiapoptosis and antifibrosis, was detected in the hADSCtreated
glands as compared to the untreated glands. Quantitative reverse transcription PCR (RT-qPCR) revealed a significantly higher expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), cyclooxygenase2 (COX2) and matrix metalloproteinase2 (MMP2)
in the hADSCtreated rats. Furthermore, immunohistochemical analysis indicated that the hADSCs had differentiated into acinar and ductal cells in the rat submandibular glands. Thus, our results suggest that hADSCs are able to regenerate irradiationdamaged salivary glands through glandular transplantation.
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Document Type: Research Article
Laboratory of Oral Biomedical Science and Translational Medicine, Department of Orthodontics, School of Stomatology, Tongji University, Shanghai 200072, P.R. China
School of Medicine, Tongji University, Shanghai 200092, P.R. China
January 1, 2014
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