Skip to main content
padlock icon - secure page this page is secure

Effects of octylphenol on the expression of cell cycle-related genes and the growth of mesenchymal stem cells derived from human umbilical cord blood

Buy Article:

$42.00 + tax (Refund Policy)

Umbilical cord blood (UCB) is defined as blood that exists in the placenta and in the attached umbilical cord following childbirth. Cord blood is now used for research purposes as it contains mesenchymal stem cells (MSCs), multipotent stromal cells which have the ability to differentiate into a variety of cell types. Among endocrine disrupting chemicals (EDCs), octylphenol (OP) is one of the alkylphenols, which are widely used industrial chemicals; these chemicals cause a number of serious side-effects, such as reproductive abnormalities. In this study, we isolated human MSCs from UCB and demonstrate that cultured MSCs express the surface marker, CD34, but not CD105. We further examined the effects of OP on human UCB-derived MSCs following exposure to OP by cell proliferation assay, semi-quantitative RT-PCR and western blot analysis. The results revealed that the transcriptional and translational levels of cyclin D1 were increased, while the levels of p21 were suppressed in the MSCs treated with OP compared with the negative controls. This collapse of the regulation of the cell cycle may directly stimulate the growth of the MSCs under culture conditions. The results from the present study provide further insight into the effects of common EDCs on MSCs derived from human UCB. However, further studies are required to identify the signaling pathways which mediate the effects of EDCs on MSCs.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: Laboratory of Veterinary Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea 2: Department of Obstetrics and Gynecology, College of Medicine, Soonchunhyang University, Bucheon, Gyeonggi 420-767, Republic of Korea 3: Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada

Publication date: January 1, 2014

More about this publication?
  • The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.

    The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Information for Advertisers
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more