@article {Huang:2013:1107-3756:1443,
title = "Electroacupuncture promotes chondrocyte proliferation via accelerated G1/S transition in the cell cycle",
journal = "International Journal of Molecular Medicine",
parent_itemid = "infobike://sp/ijmm",
publishercode ="sp",
year = "2013",
volume = "31",
number = "6",
publication date ="2013-01-01T00:00:00",
pages = "1443-1448",
itemtype = "ARTICLE",
issn = "1107-3756",
eissn = "1791-244X",
url = "https://www.ingentaconnect.com/content/sp/ijmm/2013/00000031/00000006/art00021",
doi = "doi:10.3892/ijmm.2013.1336",
author = "Huang and Wu and Fan and Ye and Liu",
abstract = "The aim of the present study was to investigate the effects of electroacupuncture (EA) on the proliferation of chondrocytes and the molecular mechanism(s) involved. Passage 2chondrocytes were randomly divided into four groups and treated with EA or nocodazole. After treatment,
cell proliferation was determined using an MTT assay and DNA staining followed by FACS. The mRNA expression levels of cyclinD1, cyclin-dependent kinase (CDK)4, CDK6, phosphorylated retinoblastoma (pRb) and P16 were detected by RT-PCR, and the protein levels of cyclinD1, CDK4, CDK6,
pRb and P16 were detected by western blotting. EA treatment significantly increased cell viability in a time-dependent manner and decreased the number of G0/G1 and G2/M phase chondrocytes and increased the number of Sphase cells. The mRNA and protein levels of cyclinD1, CDK4, CDK6,
(p)Rb and P16 consistently demonstrated a reverse trend with the levels in the chondrocytes treated with nocodazole. The expression levels of cyclinD1, CDK4, CDK6 and Rb were higher in chondrocytes receiving EA treatment when compared to levels in the untreated cells while expression
of P16 was lower. In conclusion, EA treatment promotes chondrocyte proliferation via promotion of G1/S checkpoint transition in the cell cycle dependent on the activity of the P16-cyclinD1-CDK4/6-pRb pathway and this may, in part, explain its clinical effect in the treatment of osteoarthritis.",
}