Skip to main content
padlock icon - secure page this page is secure

Millimeter wave treatment promotes chondrocyte proliferation by upregulating the expression of cyclin-dependent kinase 2 and cyclin A

Buy Article:

$42.00 + tax (Refund Policy)

We investigated the effects of millimeter wave treatment on the expression of the cell cycle regulating proteins cyclin-dependent kinase 2 (CDK2) and cyclin A in chondrocytes. Knee articular cartilage from SD rats was used to establish cultured primary chondrocytes. After identification using toluidine blue staining, passage 2 chondrocytes were randomly divided into different groups and treated with nocodazole or millimeter wave. The RNA expression of CDK2 and cyclin A was measured using RT-PCR, and their protein levels were detected by Western blotting. Cell cycle analysis showed that nocodazole treatment significantly increased the number of G0/G1 and G2/M stage chondrocytes and decreased the amount of S phase cells. In contrast, millimeter wave treatment significantly decreased the number of G0/G1 and G2/M chondrocytes and increased the number of S phase cells. The mRNA and protein levels of CDK2 and cyclin A consistently demonstrated a reverse trend, with the lowest levels in the chondrocytes treated with nocodazole. The expression of CDK2 and cyclin A was higher in chondrocytes receiving millimeter wave treatment than in untreated cells. In conclusion, millimeter wave treatment induces CDK2 and cyclin A expression, accelerates S-phase entry and G2/M transition and promotes chondrocyte cell cycle progression.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Affiliations: Fujian University of Traditional Chinese Medicine, University Town, Minhou Shangjie, Fujian 350108, P.R. China

Publication date: July 1, 2010

More about this publication?
  • The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.

    The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Information for Advertisers
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more