Anti-proliferation effect of BMI-1 in U937 cells with siRNA
Polycomb protein BMI-1 is often overexpressed in various types of leukemia, and its depletion in those leukemic cells leads to cell proliferation arrest, differentiation, and apoptosis. These findings have led us to believe that Bmi-1 has potential as a therapeutic target for leukemia. In this study, we tested multiple sets of synthetic siRNA oligo pairs for downregulating the expression of Bmi-1 in U937 cells, and explored the possible biological effects of BMI-1 suppression on cell growth and proliferation. We found that one siRNA oligo pair can efficiently knock down BMI-1 in U937 cells as evaluated by real-time RT-PCR as well as Western blot analysis. Furthermore, we found that BMI-1 depletion leads to reduced cell growth and proliferation, and increased cell apoptosis. Our proof-of-principle findings confirm that Bmi-1 siRNA can be used as a therapeutic target of any Bmi-1 expressing leukemia and other cancer cells.
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Document Type: Research Article
Affiliations: School of Medical Science and Laboratory Medicine, Jiangsu University, Jiangsu 212013, P.R. China
Publication date: June 1, 2010
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- The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.
The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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