In vitro preparation and characterization of the human CD3εε homodimer and CD3εγ and CD3εδ heterodimers
The CD3 molecule is a critical component of both humoral and cellular immune responses, and yet while the structure and molecular assembly of other key mediators such as CD4 and CD8 have been reported, individual CD3 subunits have not been well characterized. Our understanding of the manner in which they interact remains limited, and the question of how many subunits are required for a functional CD3 molecular complex is yet to be addressed. It has been suggested that CD3ε pairs with CD3γ or with CD3δ, forming CD3εγ and CD3εδ heterodimers that associate with α/β T cell receptors (TCRs) and CD3 ζ 2 dimers. In this study we investigated whether interactions between each CD3ε subunit play a role in the formation of the CD3 molecular complex. Our results revealed that the human CD3ε subunit forms a homodimer structure, which is a crucial piece of information for the elucidation of cellular signaling following TCR receptor ligation, and provide insight into our understanding of the molecular assembly of the CD3 molecular complex.
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Document Type: Research Article
Affiliations: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China
Publication date: January 1, 2009
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- The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.
The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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