Metabolic effects of X-ray irradiation on adult human articular chondrocytes
There have been few reports regarding the metabolic effects of X-ray irradiation on adult human articular chondrocytes. The purpose of this study was to evaluate whether exposure to X-ray irradiation during tumor surgery can cause impaired metabolism in adult articular cartilage. To achieve this we exposed cultured chondrocytes isolated from normal or degenerated cartilage to varying doses of X-ray irradiation, then measured apoptosis, and the production of chondroitin sulfates (CS), prostaglandin E2 (PGE2) and p38 mitogen-activated protein kinase (MAPK) in these cells. The number of apoptotic cells was not affected by irradiation in chondrocytes from normal or degenerated cartilage. Likewise, the production of C6S and C4S was not altered by irradiation in either group. The concentration of PGE2 in non-degenerated chondrocyte cultures did not change with radiation in a dose-dependent manner. However, the concentration of PGE2 in degenerated chondrocytes increased in a radiation dose-dependent manner. Irradiation at 10 Gy, in degenerated chondrocytes, induced remarkable activation of p38. This suggests that it is important to consider whether there is an osteoarthritic joint in the area that is to receive radiation therapy during tumor surgery.
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Document Type: Research Article
Affiliations: Department of Orthopaedic Surgery, Kochi Medical School, Oko-cho, Nankoku, Kochi 783-8505, Japan., Email: [email protected]
Publication date: May 1, 2003
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- The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.
The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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