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Phospholipase C-γ1 is required for subculture-induced terminal differentiation of normal human oral keratinocytes

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Serial subculture of primary normal human oral keratinocytes (NHOKs) to the post-mitotic stage induces terminal differentiation, which is in part linked to elevated levels of phospholipase C (PLC)-γ1. Therefore, PLC-γ1 may be involved in the signal transduction system that leads to the calcium regulation of subculture-induced keratinocyte differentiation. To test this hypothesis, the expression of PLC-γ1 in primary NHOKs was blocked by transfecting cells with the antisense PLC-γ1 cDNA construct. These cells demonstrated dramatic reductions in PLC-γ1 protein and in the differentiation markers involucrin and transglutaminase following calcium exposure and an increase (15≈20%) in in vitro life span versus empty vector-transfected cells. In addition, we established the ability of antisense PLC-γ1 to block the serial subculture-induced rise in intracellular calcium. Similar observations were made following treatment with the specific PLC inhibitor U73122. These results indicate that the terminal differentiation of NHOKs by serial subculture is associated with PLC-γ1, which mediates calcium regulation by mobilizing intracellular calcium.
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Document Type: Research Article

Affiliations: Department of Oral Biochemistry, Dental Research Institute, IBEC, and BK21 HLS, Seoul National University, College of Dentistry, Seoul, Korea

Publication date: January 1, 2003

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  • The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.

    The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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