Additive effects of amyloid β fragment and interleukin-1β on interleukin-6 secretion in rat primary glial cultures
The main constituent of the Alzheimer amyloid plaques is the amyloid β (Aβ) peptide shown to activate glial cells in vitro. Activated glial cells are believed to contribute to neurotoxicity through production of inflammatory cytokines, such as interleukin-1 (IL-1), chemokines and neurotoxic substances. The IL-1 system has been proposed to play a role in neurodegenerative processes and can in turn induce expression of other cytokines such as IL-6. Recently, association of IL-1 and IL-6 gene polymorphism with Alzheimer's disease was reported, suggesting that these cytokines may play an important role in the development of the disease. In this study, rat primary mixed glial cells were treated with IL-1β, Aβ(1-42) or Aβ(25-35). As expected the different treatments all resulted in activation of the transcription factor NFκB observed by electrophoretic mobility shift assay. Significant increases in IL-1β and IL-6 mRNA levels, as analysed by reverse transcriptase-polymerase chain reaction (RT-PCR), were detected after the different treatments. In addition, increased secretion of IL-6 was detected by ELISA after 96 h exposure in response to IL-1β, Aβ(1-42) or Aβ(25-35). When cells were exposed to both IL-1β and Aβ(25-35) additive effects were observed. This supports that the effect of Aβ can be potentiated by concurrent exposure to inflammatory cytokines and that the IL-1 system is not necessary for Aβ effects on IL-6 expression in agreement with previous studies.
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Document Type: Research Article
Affiliations: Department of Neurochemistry and Neurotoxicology, The Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden
Publication date: January 1, 2002
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- The International Journal of Molecular Medicine is a monthly, peer-reviewed journal devoted to the publication of high quality studies related to the molecular mechanisms of human disease. The journal welcomes research on all aspects of molecular and clinical research, ranging from biochemistry to immunology, pathology, genetics, human genomics, microbiology, molecular pathogenesis, molecular cardiology, molecular surgery and molecular psychology.
The International Journal of Molecular Medicine aims to provide an insight for researchers within the community in regard to developing molecular tools and identifying molecular targets for the diagnosis and treatment of a diverse number of human diseases.
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