miR1505p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR
Dysregulated microRNAs (miRNAs or miRs) serve potential roles in inflammatory systemic disease, including ankylosing spondylitis (AS). The aim of the present study was to investigate the potential function of miR1505p in osteogenic differentiation of AS fibroblasts and its underlying
mechanism. The expression of miR1505p and vitamin D receptor (VDR) in AS joint capsules and fibroblasts was detected by reverse transcriptionquantitative (RTq)PCR and western blotting. Following overexpression of miR1505p, the alteration in osteogenic gene expression was detected by RTqPCR,
western blotting and alkaline phosphatase activity assay, as well as alizarin red staining. The association between miR1505p and VDR was confirmed by luciferase assay and rescue experiments were performed. Patients with AS exhibited decreased expression of miR1505p in joint capsules. Treatment
with bone morphogenic protein 2 (BMP2) and transforming growth factor β1 (TGFβ1) led to downregulation of miR1505p in AS fibroblasts. Enforced expression of miR1505p attenuated osteogenic differentiation of AS fibroblasts. These results demonstrated that miR1505p inhibited osteogenic
differentiation of AS fibroblasts by targeting VDR. miR1505p overexpression decreased osteogenic transformation of fibroblasts by decreasing VDR expression in AS.
Document Type: Research Article
Affiliations: Department of Rheumatology and Immunology, Tianjin First Central Hospital, Tianjin 300192, P.R. China
Publication date: 01 January 2022
- Experimental and Therapeutic Medicine aims to ensure the expedient publication, in both print and electronic format, of studies relating to biology, gene therapy, infectious disease, microbiology, molecular cardiology and molecular surgery. The journal welcomes studies pertaining to all aspects of molecular medicine, and studies relating to in vitro or in vivo experimental model systems relevant to the mechanisms of disease are also included.
All materials submitted to this journal undergo the appropriate review via referees who are experts in this field. All materials submitted follow international guidelines with regard to approval of experiments on humans and animals. - Editorial Board
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