The effect of bone marrow mesenchymal stem cells on highly metastatic MHCC97H hepatocellular carcinoma cells following OPN and TGFβ1 gene silencing
The metastatic behavior of hepatocellular carcinoma (HCC) is one of the key factors that leads to poor prognosis. The aim of the current study was to determine the changes in metastasis and the proliferation potential of bone marrow mesenchymal stem cells (BMSCs) in high metastatic potential hepatocellular carcinoma (MHCC97H) following gene silencing. The osteopontin (OPN) and transforming growth factorβ (TGFβ1) genes, which are associated with metastasis and tumor proliferation, were silenced in MHCC97H cells. Transwell assays were used to evaluate the migration of MHCC97H cells in vitro. Additionally, a murine model of MHCC97H lung metastasis was established. Following OPN and TGFβ1 silencing, the migration of MHCC97H cells was significantly reduced following BMSC intervention (P<0.01). Furthermore, there were few MHCC97H cells in the lung tissues of the OPN and TGFβ1silenced animals, and their integrated optical density (IOD) value was significantly lower compared with controls (P<0.05). Immunofluorescence of lung metastasis in the MHCC97H model revealed that there was no significant difference in the IOD value of integrin αvβ3 expression in the OPN and TGFβ1silenced groups compared with controls (P>0.05). The metastasis and proliferation potential of MHCC97H following BMSC intervention were significantly reduced in vitro and in vivo, especially in the TGFβ1silenced group. The decrease in the metastatic potential in genesilenced MHCC97H cells was not associated with integrin αvβ3 expression. Therefore, OPN and TGFβ1 may be potential targets for HCC treatment, and TGFβ1 may have a higher therapeutic potential for BMSC intervention.
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Document Type: Research Article
Affiliations: Department of Radiology, the Fourth Medical Center of Chinese PLA General Hospital, Beijing 100048, P.R. China
Publication date: October 1, 2020
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- Experimental and Therapeutic Medicine aims to ensure the expedient publication, in both print and electronic format, of studies relating to biology, gene therapy, infectious disease, microbiology, molecular cardiology and molecular surgery. The journal welcomes studies pertaining to all aspects of molecular medicine, and studies relating to in vitro or in vivo experimental model systems relevant to the mechanisms of disease are also included.
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