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Phosphorylation of PRAS40 contributes to the activation of the PI3K/AKT/mTOR signaling pathway and the inhibition of autophagy following status epilepticus in rats

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Status epilepticus (SE) is a neurological disorder associated with high morbidity and mortality rates, and is often difficult to treat. Moreover, the underlying mechanism of SE remains unknown. The lithiumpilocarpine model is a validated animal model that can reproduce the main clinical and neuropathological features of SE. In the present study, this SE model was utilized and SE was successfully established in rats, as determined by the corresponding epileptic electroencephalogram. Histology, immunohistochemistry, western blot analysis and coimmunoprecipitation were used to detect the phosphorylation (p) of AKT substrate of 40 kDa (PRAS40), the combination of pPRAS40 and 1433 protein and the activation of the PI3K/mTOR signaling pathway in SE. In addition, the present study analyzed the dynamics of the expression of autophagyassociated factors in the hippocampus after SE induction, and the influence of suppressing the p of PRAS40 on the autophagy process was detected in the pathogenesis of SE. The results indicated that increased pPRAS40 expression could activate the mTOR pathway to decrease the level of autophagy. However, inhibition of the mTOR signaling pathway promoted autophagy flux. These results may provide further understanding of pPRAS40 functions in SE.
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Document Type: Research Article

Affiliations: 1: School of Policing Studies, Shanghai University of Political Science and Law, Shanghai 201701, P.R. China 2: General Family Medicine Clinic, Xuhui District Xietu Subdistrict Community Healthcare Center, Shanghai 200120, P.R. China 3: Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China 4: Institute of Forensic Science, Jiading Branch of Shanghai Public Security Bureau, Shanghai 201800, P.R. China 5: Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China

Publication date: October 1, 2020

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  • Experimental and Therapeutic Medicine aims to ensure the expedient publication, in both print and electronic format, of studies relating to biology, gene therapy, infectious disease, microbiology, molecular cardiology and molecular surgery. The journal welcomes studies pertaining to all aspects of molecular medicine, and studies relating to in vitro or in vivo experimental model systems relevant to the mechanisms of disease are also included.

    All materials submitted to this journal undergo the appropriate review via referees who are experts in this field. All materials submitted follow international guidelines with regard to approval of experiments on humans and animals.
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