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LncRNA NEAT1 promotes apoptosis and inflammation in LPSinduced sepsis models by targeting miR5903p

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Sepsis is a complication of infection caused by disease or trauma. Increasing evidence have shown that long noncoding RNAs (lncRNAs) are involved in the regulation of sepsis. However, the mechanism of lncRNA nuclear enriched abundant transcript 1 (NEAT1) in the regulation of sepsis progression remains to be elucidated. Lipopolysaccharide (LPS) was used to induce a sepsis cell model. The expression levels of NEAT1 and microRNA (miR)5903p were determined by reverse transcriptionquantitative PCR. Cell viability and apoptosis were detected using Cell Counting Kit8 (CCK8) assay and flow cytometry, respectively. Western blot analysis was performed to evaluate the levels of apoptosis and NFκB signaling pathwayrelated proteins. The concentration of inflammatory cytokines was determined using ELISA. In addition, dualluciferase reporter assay, RNA immunoprecipitation and biotinlabeled RNA pulldown assay were performed to verify the interaction between NEAT1 and miR5903p. The results showed that NEAT1 was highly expressed in patients with sepsis and LPSinduced H9c2 cells. Knockdown of NEAT1 decreased LPSinduced cell apoptosis and inflammation response in H9c2 cells. Meanwhile, miR5903p showed decreased expression in sepsis, and its overexpression could relieve LPSinduced H9c2 cell damage. Further experiments revealed that NEAT1 could sponge miR5903p. Knockdown of miR5903p reversed the inhibitory effect of NEAT1 knockdown on LPSinduced H9c2 cell damage. Additionally, the NEAT1/miR5903p axis could regulate the activity of the NFκB signaling pathway. To conclude, lncRNA NEAT1 accelerated apoptosis and inflammation in LPSstimulated H9c2 cells via sponging miR5903p. These findings may provide a new strategy for the treatment of sepsis.
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Document Type: Research Article

Affiliations: 1: Emergency Department, First Affiliated Hospital of the University of South China, Hengyang, Hunan 421001, P.R. China 2: Center of Functional Laboratory, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China

Publication date: October 1, 2020

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  • Experimental and Therapeutic Medicine aims to ensure the expedient publication, in both print and electronic format, of studies relating to biology, gene therapy, infectious disease, microbiology, molecular cardiology and molecular surgery. The journal welcomes studies pertaining to all aspects of molecular medicine, and studies relating to in vitro or in vivo experimental model systems relevant to the mechanisms of disease are also included.

    All materials submitted to this journal undergo the appropriate review via referees who are experts in this field. All materials submitted follow international guidelines with regard to approval of experiments on humans and animals.
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