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miR29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castrationinduced osteoporosis through the PI3K/AKT and TGFβ/Smad signaling pathways

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The aim of the present study was to investigate the role of microRNA (miR)-29b in the proliferation and migration of bone marrow mesenchymal stem cells (BMSCs) in rats with castrationinduced osteoporosis and the relevant mechanisms. The gene expression profiling microarray technique was utilized to sequence the BMSCs with overexpressed miR29b. The intersection of the potential targets and the genes downregulated in the sequencing were utilized for GO enrichment analysis. Gene set enrichment analysis (GSEA) was employed to analyze the effect of miR29b on signaling pathways. Additionally, the effects of miR29b overexpression on the phosphatidylinositol 3kinase (PI3K)/protein kinase B (AKT) and transforming growth factorβ (TGFβ)/Drosophila mothers against decapentaplegic protein (Smad) signaling pathways were detected via RTqPCR assay and western blotting. The expression level of miR29b was found to be significantly reduced in bone marrow tissues of postmenopausal osteoporosis patients and BMSCs of rats with castrationinduced osteoporosis established via ovariectomy. Based on transcriptome sequencing and bioinformatics software prediction, 76 potential targets of miR29b were obtained, which were distinctly enriched in such biological processes as cell proliferation, cell cycle, cell migration and cell adhesion. The results of CCK8 and EdU assays showed that overexpression of miR29b overtly promoted the proliferation of BMSCs in rats with castrationinduced osteoporosis. Moreover, the Transwell assay results revealed that the overexpression of miR29b significantly facilitated the migration of BMSCs in rats with castrationinduced osteoporosis. According to RTqPCR assay and western blotting, miR29b activated the PI3K/AKT and TGFβ/Smad signaling pathways. miR29b exhibited a clearly lower expression level in the bone marrow tissues of the postmenopausal osteoporosis patients and BMSCs of rats with castrationinduced osteoporosis established via ovariectomy. Overexpression of miR29b was able to enhance the proliferation and migration ability of BMSCs in rats with castrationinduced osteoporosis, and such an enhancement may be correlated with the activation of the PI3K/AKT and TGFβ/Smad signaling pathways.
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Document Type: Research Article

Affiliations: 1: Department of Clinical Laboratory, Affiliated Hospital of Beihua University, Jilin, Jilin 132011, P.R. China 2: Department of Clinical Laboratory, Jinan Zhangqiu District Hospital of TCM, Jinan, Shandong 250200, P.R. China 3: Department of Orthopaedics, Qingdao Central Hospital, Qingdao University, Qingdao, Shandong 266042, P.R. China 4: Department of Rehabilitation, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China 5: Department of CardioThoracic Surgery, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China 6: Department of Clinical Laboratory, Qingdao No. 9 People's Hospital, Qingdao, Shandong 266002, P.R. China

Publication date: October 1, 2020

More about this publication?
  • Experimental and Therapeutic Medicine aims to ensure the expedient publication, in both print and electronic format, of studies relating to biology, gene therapy, infectious disease, microbiology, molecular cardiology and molecular surgery. The journal welcomes studies pertaining to all aspects of molecular medicine, and studies relating to in vitro or in vivo experimental model systems relevant to the mechanisms of disease are also included.

    All materials submitted to this journal undergo the appropriate review via referees who are experts in this field. All materials submitted follow international guidelines with regard to approval of experiments on humans and animals.
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