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MicroRNA652 promotes cell proliferation and osteosarcoma invasion by directly targeting KLF9

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Previous studies have demonstrated that various microRNAs (miRNAs or miRs) are abnormally expressed in osteosarcoma (OS) and serve roles in its malignant development. An indepth understanding of the specific roles of dysregulated miRNAs in OS may be important for cancer research and the identification of novel therapeutic targets. In the current study, reverse transcriptionquantitative PCR was performed to determine miR652 expression in OS tissues and cell lines. Cell Counting Kit8 and Transwell invasion assays were used for assessing the effect of miR652 on the proliferation and invasion of OS cells. Herein, miR652 expression was assessed in OS and the effects and molecular mechanisms of miR652 in OS cells were examined. The results revealed that miR652 expression was significantly upregulated in OS tissues and cell lines compared with adjacent normal tissues and a normal human osteoblast cell line. Furthermore, miR652 downregulation inhibited the proliferation and invasion of OS cells. miR652 was also demonstrated to directly interact with the 3'untranslated region of kruppellike factor 9 (KLF9) and miR652 negatively regulated KLF9 expression in OS cells. miR652 and KLF9 mRNA levels were also revealed to be inversely correlated in OS tissues. Treatment with KLF9 small interfering RNA abolished the suppression of OS proliferation and invasion induced by miR652 downregulation. miR652 may serve an oncogenic role in OS cells by targeting KLF9 directly. The results also indicated that miR652 may be an effective novel therapeutic target for the treatment of patients with OS.
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Document Type: Research Article

Affiliations: 1: Institute of Nursing Allied Health Sciences, College of Nursing and Health Science, Henan University, Kaifeng, Henan 475001, P.R. China 2: Department of Nursing, Kaifeng Health School, Kaifeng, Henan 475001, P.R. China

Publication date: October 1, 2020

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  • Experimental and Therapeutic Medicine aims to ensure the expedient publication, in both print and electronic format, of studies relating to biology, gene therapy, infectious disease, microbiology, molecular cardiology and molecular surgery. The journal welcomes studies pertaining to all aspects of molecular medicine, and studies relating to in vitro or in vivo experimental model systems relevant to the mechanisms of disease are also included.

    All materials submitted to this journal undergo the appropriate review via referees who are experts in this field. All materials submitted follow international guidelines with regard to approval of experiments on humans and animals.
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