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Lung injury caused by paraquat poisoning results in increased interleukin6 and decreased microRNA146a levels

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The aim of the present study was to investigate the expression of microRNA (miR)146a in the pulmonary macrophages, peripheral blood mononuclear cells and serum of patients with lung injury caused by paraquat poisoning, as well as the underlying mechanism of its regulation in the disease. A total of 26 patients with lung injury caused by paraquat poisoning were included in the present study. In addition, 33 healthy subjects were included as the control group. The expression levels of interleukin (IL)6 mRNA and miR146a was determined by reverse transcriptionquantitative polymerase chain reaction (RTqPCR). Western blotting was used to measure IL6 protein expression, while enzymelinked immunosorbent assay was also performed to determine the secretion of IL6 protein. A dualluciferase reporter assay was conducted to examine whether IL6 mRNA is a direct target of miR146a. Patients with lung injury caused by paraquat poisoning exhibited higher IL6 mRNA and protein levels as compared with those in healthy subjects. In addition, miR146a expression in patients with paraquat poisoninginduced lung injury was significantly reduced in comparison with that in healthy subjects. Notably, the overexpression of miR146a by mimic transfection downregulated the expression of IL6 in pulmonary macrophages. The results of dualluciferase reporter assay demonstrated that IL6 mRNA was a direct target of miR146a. Therefore, the present study demonstrated that increased expression of IL6 in patients with lung injury caused by paraquat poisoning is associated with decreased expression of miR146a. Furthermore, miR146a may regulate the occurrence and immune response of lung injury caused by paraquat poisoning and this process is possibly achieved via IL6, an important cytokine that mediates inflammation.
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Document Type: Research Article

Affiliations: 1: Department of Respiratory Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, P.R. China 2: Emergency Department, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, P.R. China

Publication date: July 1, 2018

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  • Experimental and Therapeutic Medicine aims to ensure the expedient publication, in both print and electronic format, of studies relating to biology, gene therapy, infectious disease, microbiology, molecular cardiology and molecular surgery. The journal welcomes studies pertaining to all aspects of molecular medicine, and studies relating to in vitro or in vivo experimental model systems relevant to the mechanisms of disease are also included.

    All materials submitted to this journal undergo the appropriate review via referees who are experts in this field. All materials submitted follow international guidelines with regard to approval of experiments on humans and animals.
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