Skip to main content
padlock icon - secure page this page is secure

Open Access Development of simple and rapid food allergen detection method and its use for on-site analysis

Download Article:
(PDF 510.1 kb)
There are currently a range of methods for detection of allergens in food in place in countries around the world, but they are not without their problems. In response to this, a researcher based at the National Institute of Health Sciences in Japan is investigating ways of improving food allergen detection methods. Dr Shinobu Sakai, Section Chief of Division of Environmental Chemistry at the Institute, is hoping to address some specific knowledge gaps in the field. 'A labelling system for food allergenic ingredients was established in Japan in April 2002. To monitor the labelling, the Japanese government announced official methods for detecting allergens in processed foods in November 2002,' explains Sakai. 'The official methods consist of quantitative screening tests using enzyme-linked immunosorbent assays (ELISAs) and qualitative confirmation tests using Western blotting or polymerase chain reactions (PCR). These official methods are carried out in laboratory sites by scientists.' Throughout the course of his studies, Sakai has already achieved notable progress, including the development of an onsite detection system by magnetic beads-binding enzyme-linked immunosorbent assays (ELISA). 'While immunoprecipitation reaction using gel carriers such as sepharose and agarose is already widely used for protein isolation and purification, the reaction is caused by nonspecific adsorption due to the surface area size,' he outlines. 'In addition, there are many problems such as high detection background due to contaminants during incubation. Then there is the fact that each step involves complicated operations such as high-speed centrifugal separation and washing, and therefore sample loss and residual insoluble matter are problems.' However, because the micro magnetic beads he has developed accumulate support using magnetic force, there is no need for centrifugal operation, and the risk of sample loss, etc. can be reduced. 'The primary antibody is bound to the hydrophilic magnetic microbead and added to the antigen solution (food extract) to capture the antigen. Next, a secondary antibody labeled with colloidal gold particles is allowed to react to perform a coloring reaction,' explains Sakai. 'Taking the convenience of detection into consideration, by mixing the food allergen extract and the lyophilised gold colloid labeled IgG probe in the same tube and accumulating the antigen-labeled antibody complex at the magnet part, it is possible to perform the detection step with one pot.' 'Moreover, by using monodisperse polystyrene microspheres incorporating various dyes inside the particles instead of colloidal gold particles, it is possible to apply the technology to the sandwich type of magnetic beads, food allergen protein and dye beads,' Sakai continues. This enables multiple detection by color tones such as red for egg (anti-ovalbumin antibody-labeled beads), blue for milk (anti-lactoglobulin antibody-labeled beads), and green for wheat (anti-gliadin antibody-labeled beads). In this particular detection method, he has developed, all steps - excluding food allergen extraction - are completed within the target time of two minutes.
No References for this article.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Affiliations: National Institute of Health Sciences, Japan

Publication date: April 1, 2019

More about this publication?
  • Impact is a series of high-quality, open access and free to access science reports designed to enable the dissemination of research impact to key stakeholders. Communicating the impact and relevance of research projects across a large number of subjects in a content format that is easily accessible by an academic and stakeholder audience. The publication features content from the world's leading research councils, policy groups, universities and research projects. Impact is published under a CC-BY Creative Commons licence.

  • Subscribe to this Title
  • Terms & Conditions
  • Disseminating research in Impact
  • Information about Impact
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more