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Open Access Analytical Approaches for Monitoring DNA–Protein Interactions

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This article is Open Access under the terms of the Creative Commons CC BY-NC-ND licence.

DNA–protein interactions play a critical role in cellular regulation. We herein review existing analytical methods for investigating these interactions, highlighting methods such as chromatin immunoprecipitation and yeast-one-hybrid that are used to identify undiscovered DNA–protein interactions. We summarize the most common approaches for characterizing known interactions based on DNA–protein structure, thermodynamic and kinetic measurements, and dynamic binding assays. We discuss techniques in optical imaging as well as representative methods, such as eletrophoretic mobility shift assay and surface plasmon resonance. The advantages and disadvantages of these techniques are used to assess a proposed optical platform based on single-walled carbon nanotube (SWCNT) fluorescence.

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Keywords: BIOSENSOR; CARBON NANOTUBE; DNA–PROTEIN INTERACTION; PHOTOLUMINESCENCE

Document Type: Research Article

Affiliations: 1: Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale of Lausanne (EPFL), Lausanne CH-1015, SCS-DSM Award for best poster presentation in Analytical Sciences 2: Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale of Lausanne (EPFL), Lausanne CH-1015;, Email: [email protected]

Publication date: April 1, 2019

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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