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Open Access Pulsed EPR Methods to Study Biomolecular Interactions

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This article is Open Access under the terms of the Creative Commons CC BY-NC-ND licence.

Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide.

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Keywords: DEER; ORTHOGONAL SPIN LABELLING; PULSED DIPOLAR SPECTROSCOPY; RIDME; SITE-DIRECTED SPIN LABELLING

Document Type: Research Article

Affiliations: 1: Laboratory of Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, CH-8093 Zurich, SCS-Metrohm Award for best oral presentation in Physical Chemistry;, Email: [email protected] 2: Laboratory of Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, CH-8093 Zurich 3: Faculty of Chemistry and Center for Molecular Materials (CM2), Bielefeld University, Universit√§tsstrasse 25, D33615 Bielefeld

Publication date: April 1, 2019

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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