Skip to main content
padlock icon - secure page this page is secure

Open Access Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET

Download Article:
 Download
(PDF 510.1 kb)
 

This article is Open Access under the terms of the Creative Commons CC BY-NC-ND licence.

Exploring the spatiotemporal dynamics of biomolecules on a single-molecule level requires innovative ways to make them spectroscopically visible. Fluorescence resonance energy transfer (FRET) uses a pair of organic dyes as reporters to measure distances along a predefined biomolecular reaction coordinate. For this nanoscopic ruler to work, the fluorescent labels need to be coupled onto the molecule of interest in a bioorthogonal and site-selective manner. Tagging large non-coding RNAs with single-nucleotide precision is an open challenge. Here we summarize current strategies in labeling riboswitches and ribozymes for fluorescence spectroscopy and FRET in particular. A special focus lies on our recently developed, DNA-guided approach that inserts two fluorophores through a stepwise process of templated functionality transfer and click chemistry.

53 References.

No Supplementary Data.
No Article Media
No Metrics

Keywords: BIOORTHOGONAL; FLUORESCENCE; NUCLEIC ACIDS; RIBOSWITCH; SPECTROSCOPY

Document Type: Research Article

Affiliations: 1: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, SCS-Metrohm Award for best oral presentation in Medicinal Chemistry & Chemical Biology;, Email: [email protected] 2: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich 3: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich;, Email: [email protected]

Publication date: April 1, 2019

More about this publication?
  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Information for Advertisers
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more