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Open Access Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET

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This article is Open Access under the terms of the Creative Commons CC BY-NC-ND licence.

Exploring the spatiotemporal dynamics of biomolecules on a single-molecule level requires innovative ways to make them spectroscopically visible. Fluorescence resonance energy transfer (FRET) uses a pair of organic dyes as reporters to measure distances along a predefined biomolecular reaction coordinate. For this nanoscopic ruler to work, the fluorescent labels need to be coupled onto the molecule of interest in a bioorthogonal and site-selective manner. Tagging large non-coding RNAs with single-nucleotide precision is an open challenge. Here we summarize current strategies in labeling riboswitches and ribozymes for fluorescence spectroscopy and FRET in particular. A special focus lies on our recently developed, DNA-guided approach that inserts two fluorophores through a stepwise process of templated functionality transfer and click chemistry.

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Document Type: Research Article

Affiliations: 1: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, SCS-Metrohm Award for best oral presentation in Medicinal Chemistry & Chemical Biology;, Email: [email protected] 2: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich 3: Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich;, Email: [email protected]

Publication date: April 1, 2019

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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