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Photochemically Active Dyes for Super-Resolution Microscopy

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The development of super-resolved optical microscopies has revolutionized the way we visualize cell biology. These techniques strongly rely on the use of photochemically active fluorophores that display changes in their photophysical properties upon irradiation with light. Many reversible and irreversible photochemical transformations have been explored for this purpose, and different imaging techniques require specific mechanisms of photoconversion. In this review, we provide an overview of the most common strategies used for the development of fluorophores for super-resolution microscopy and give specific examples of state-of-the-art fluorogenic probes. Furthermore, we discuss their main field of application and possible directions for future developments.
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Keywords: FLUORESCENT PROBES; LIVE-CELL IMAGING; PHOTOACTIVATABLE; PHOTOSWITCHABLE; SUPER-RESOLUTION MICROSCOPY

Document Type: Research Article

Affiliations: 1: Laboratorium für Organische Chemie ETH Zurich Vladimir-Prelog-Weg 3, CH-8093 Zurich 2: Laboratorium für Organische Chemie ETH Zurich Vladimir-Prelog-Weg 3, CH-8093 Zurich;, Email: [email protected]

Publication date: 01 November 2018

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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