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Open Access Surveying the Delivery Methods of CRISPR/Cas9 for ex vivo Mammalian Cell Engineering

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The simplicity of the CRISPR/Cas9 technology has been transformative in making targeted genome editing accessible for laboratories around the world. However, due to the sheer volume of literature generated in the past five years, determining the best format and delivery method of CRISPR/Cas9 components can be challenging. Here, we provide a brief overview of the progress that has been made in the ex vivo genome editing of mammalian cells and summarize the key advances made for improving efficiency and delivery of CRISPR/Cas9 in DNA, RNA, and protein form. In particular, we highlight the delivery of Cas9 components to human cells for advanced genome editing applications such as large gene insertion.
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Keywords: CAS9; CRISPR; GENOME EDITING

Document Type: Research Article

Affiliations: 1: Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland; National Centre of Competence in Research (NCCR), Molecular Systems Engineering, Switzerland 2: National Centre of Competence in Research (NCCR), Molecular Systems Engineering, Department of Organic Chemistry, University of Geneva, Geneva, Switzerland 3: Department of Biosystems Science and Engineering ETH Zürich, Basel, Switzerland; National Centre of Competence in Research (NCCR), Molecular Systems Engineering, Switzerland. [email protected]

Publication date: 01 June 2016

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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