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Open Access Construction of a Peptide Microarray for Auto-anti- body Detection

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Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).

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Keywords: BARD1; CLICK CHEMISTRY; MICROARRAY; PEPTIDE ANTIGENS; TUMOR AUTO-ANTIBODIES

Document Type: Research Article

Affiliations: 1: HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland 2: Institute of Chemistry, University of Applied Sciences Western Switzerland, Freiburg, Perolles 80, CH-1705 Freiburg, Switzerland 3: Institute of Sciences and Industrial Technologies, University of Applied Sciences Western Switzerland, Geneva, 4 rue de la Prairie, CH-1202 Geneva, Switzerland 4: HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland. [email protected]

Publication date: October 1, 2012

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.



    As of December 31, 2021, CHIMIA International Journal for Chemistry will no longer be hosted on Ingenta Connect. Please contact the publisher directly for more information.
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