Skip to main content
padlock icon - secure page this page is secure

Monoxenic cultures of light sensitive arbuscular mycorrhizal fungi with Lunularia cruciata (Marchantiopsida)

Buy Article:

$39.00 + tax (Refund Policy)

This study presents a new in vitro method to culture light-sensitive arbuscular mycorrhizal fungi with Lunularia cruciata. Mycothalli inoculated with Glomus clarum and Gigaspora margarita were successfully cultivated on 150 mL of dual-layer medium composed of macro- and micro-nutrients and without any of the growth promoters usually found in root organ cultures. The upper layer had the addition of 2 g L-1 of activated charcoal. Mature cultures of both fungi were obtained in less than 150 days and could be maintained viable for more than 500 days, with container size being the limiting factor for plant growth. Lunularia cruciata colonisation by both fungi showed the characteristic Paris -type morphology and was more abundant within the mycothalli's central midrib parenchyma cells than in other cells. For Gi. margarita the thallus colonisation was relatively small and shallow, suggesting a discrete distribution of the fungus within the plant and that penetration occurs mainly through new entry points of external hyphae, i.e., thallus-to-thallus penetration via appressorium. Conversely, for Gl. clarum higher colonisation was probably due to internal fungal growth along the midrib parenchyma, towards the new formed cells on the thallus' apical meristem.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: GLOMEROMYCETES; GLOMEROMYCOTA; MARCHANTIALES; MUTUALISTIC SYMBIOSIS

Document Type: Research Article

Publication date: February 1, 2014

More about this publication?
  • Nova Hedwigia is an international journal publishing original, peer-reviewed papers on current issues of taxonomy, morphology, ultrastructure and ecology of all groups of cryptogamic plants, including cyanophytes/cyanobacteria and fungi. The half-tone plates in Nova Hedwigia are known for their high quality, which makes them especially suitable for the reproduction of photomicrographs and scanning and transmission electron micrographs.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more