DNA microchips for phytoplankton: The fluorescent wave of the future
The development of DNA-chip-technology has made it possible to analyse large numbers of probes in just one experiment. We show here the results of our first DNA-chip for the identification of phytoplankton. A set of previously published probes was chosen to be tested for their use on DNA-chips. Initially the probes were developed for other hybridization-techniques, such as dotblot-hybridization or FISH (fluorescent in situ hybridization). The probes target the 18S-rDNA of phytoplankton at the level of division or class. With the resulting DNA-chip, it was possible to identify specifically Prymnesiophyta, Chlorophyta, and the heterokont algal class Bolidophyceae. Our results indicate that DNA-chip-technology has great potential in phytoplankton analysis.
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Document Type: Research Article
Publication date: August 1, 2004
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- Nova Hedwigia is an international journal publishing original, peer-reviewed papers on current issues of taxonomy, morphology, ultrastructure and ecology of all groups of cryptogamic plants, including cyanophytes/cyanobacteria and fungi. The half-tone plates in Nova Hedwigia are known for their high quality, which makes them especially suitable for the reproduction of photomicrographs and scanning and transmission electron micrographs.
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