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Using fluorescently-labelled rRNA probes for hierarchical estimation of phytoplankton diversity – a mini-review

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Molecular probes can be used to identify and characterize phytoplankton species by DNA dot blot or in situ hybridization with subsequent detection by fluorescence microscopy or flow cytometry. More or less conserved regions on the rRNA genes make it possible to develop probes that are specific for different taxonomic levels from higher groups, like eukaryotes, down to species and even strain levels. These hierarchical probes can be used to estimate biodiversity at a variety of taxonomic levels and are a valuable tool for analysing microalgae and answering ecological questions.
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Document Type: Research Article

Publication date: August 1, 2004

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  • Nova Hedwigia is an international journal publishing original, peer-reviewed papers on current issues of taxonomy, morphology, ultrastructure and ecology of all groups of cryptogamic plants, including cyanophytes/cyanobacteria and fungi. The half-tone plates in Nova Hedwigia are known for their high quality, which makes them especially suitable for the reproduction of photomicrographs and scanning and transmission electron micrographs.
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