Skip to main content
padlock icon - secure page this page is secure

First attempts to cryopreserve strains from the Coimbra Collection of Algae (ACOI) and the use of image analysis to assess viability

Buy Article:

$39.00 + tax (Refund Policy)

Serial sub-culture has always been the principle method for maintaining the cultures of the Coimbra Collection of Algae (ACOI). This technique is time consuming, material and human resources demanding, involves the risk of strain loss and could potentially lead to genetic changes over time. The preservation of cultures at ultra-low temperatures has recently become possible at Coimbra due to the acquisition and establishment of a cryopreservation unit under the ongoing European funded COBRA project (http://www. cobra. ac. uk ). About 100 ACOI strains have been frozen using a standard two-step protocol. The protocol employed dimethyl sulphoxide (DMSO), glycerol and methanol as cryoprotectants in an uncontrolled 2-step cooling process, followed by plunging into liquid nitrogen. The majority of the strains cryopreserved belong to the Chlorococcales, although representatives of the Cyanobacteria, Eustigmatophyceae, Glaucophyceae, Volvocales, Tetrasporales, Chaetophorales, Trebouxiophyceae and Desmidiales were also tested. Some strains within the Chlorococcales and the Eustigmatophyceae demonstrated 100% viability after the treatment; however, no viability was observed for the Tetrasporales and Desmidiales species examined. Viabilities of some strains were compared after cryopreservation in the presence of the three different cryoprotectants and employing a range of intermediate freeze temperatures. DMSO and methanol were the most frequently used cryoprotectants with samples cooled to an intermediate temperature at -80°C. In order to assess strain viability, a new approach based on image analysis of algal colony counts on agar plates was developed. From the data obtained it appears that each strain has its own unique optimal freezing condition, and even closely related strains have different optimum cryopreservation conditions.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: August 1, 2004

More about this publication?
  • Nova Hedwigia is an international journal publishing original, peer-reviewed papers on current issues of taxonomy, morphology, ultrastructure and ecology of all groups of cryptogamic plants, including cyanophytes/cyanobacteria and fungi. The half-tone plates in Nova Hedwigia are known for their high quality, which makes them especially suitable for the reproduction of photomicrographs and scanning and transmission electron micrographs.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more