@article {Orihara:2016:0031-5850:173,
title = "Evolutionary history of the sequestrate genus Rossbeevera (Boletaceae) reveals a new genus Turmalinea and highlights the utility of ITS minisatellite-like insertions for molecular identification",
journal = "Persoonia - Molecular Phylogeny and Evolution of Fungi",
parent_itemid = "infobike://nhn/pimj",
publishercode ="nhn",
year = "2016",
volume = "37",
number = "1",
publication date ="2016-12-28T00:00:00",
pages = "173-198",
itemtype = "ARTICLE",
issn = "0031-5850",
eissn = "1878-9080",
url = "https://www.ingentaconnect.com/content/nhn/pimj/2016/00000037/00000001/art00009",
doi = "doi:10.3767/003158516X691212",
keyword = "INTROGRESSION, SPECIES TREE, DNA BARCODING, HYPOGEOUS FUNGI, BIOGEOGRAPHY, CRYPTIC SPECIES",
author = "Orihara, T. and Lebel, T. and Ge, Z.-W. and Smith, M.E. and Maekawa, N.",
abstract = "The sequestrate (truffle-like) basidiomycete genera Rossbeevera, Chamonixia, and Octaviania are closely related to the epigeous mushroom genera Leccinum and Leccinellum. In order to elucidate the properties and placement of several undescribed sequestrate
taxa in the group and to reveal the evolutionary history of Rossbeevera and its allies, we conducted phylogenetic analyses based on three nuclear (ITS, nLSU, EF-1) and two mitochondrial DNA loci (ATP6 and mtSSU) as well as precise morphological observations. Phylogenetic
analyses of three nuclear loci suggest a complex evolutionary history with sequestrate fruiting bodies present in several clades, including a previously unrecognized sister clade to Rossbeevera. Here we propose a new sequestrate genus, Turmalinea, with four new species and one
new subspecies as well as two new species of Rossbeevera. The three-locus nuclear phylogeny resolves species-level divergence within the Rossbeevera-Turmalinea lineage, whereas a separate phylogeny based on two mitochondrial genes corresponds to geographic distance within each
species-level lineage and suggests incomplete lineage sorting (ILS) and gene introgression within several intraspecific lineages of Rossbeevera. Furthermore, topological incongruence among the three nuclear single-locus phylogenies suggests that ancient speciation within Rossbeevera
probably involved considerable ILS. We also found an unusually long, minisatellite-like insertion within the ITS2 in all Rossbeevera and Turmalinea species. A barcode gap analysis demonstrates that the insertion is more informative for discrimination at various taxonomic levels
than the rest of the ITS region and could therefore serve as a unique molecular barcode for these genera.",
}