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mRNA expression of nuclear receptor RZR/RORα, melatonin membrane receptor MT1, and hydroxindole-O-methyltransferase in different populations of human immune cells

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We characterized the expression levels of the retinoid Z receptor α (RZR α), RORα mRNA isoforms (RORα1, RORα2, and RORα3), and both melatonin receptor MT1 and hydroxindole-O-methyltransferase (HIOMT) genes. For this purpose, the following human peripheral blood mononuclear cells populations were isolated: monocytes (CD14+ cells), B lymphocytes (CD19+ cells), T helper lymphocytes (CD14 CD4+), cytotoxic T lymphocytes (CD56 CD8+ cells), and natural killer (NK) lymphocytes (CD56+ cells). PBMCs subsets were obtained by Dynabeads M-450 (Dynal) isolation procedure. We observed a strong gene expression signal for RZRα in all subpopulations studied, whereas both RORα1 and RORα2 transcripts were amplified only in CD8+ cells. Specific signal for RORα2 was obtained in all subpopulations studied, but we were not able to detect the RORα3 mRNA transcript in human immune cells studied. A weaker signal (especially in CD19+ cells) was also detected in all subsets of cells for the MT1 gene. With regard to HIOMT, a strong signal was achieved among all but one subpopulation of cells; the only exception was CD14+ cells. Thus, in addition to its classical function in the nervous and endocrine system, melatonin could act directly as a paracrine and/or autocrine agent in the human immune system.
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Keywords: DNA-binding protein; G protein-coupled receptor; gene expression; melatonin; neuroimmunology

Document Type: Research Article

Publication date: August 1, 2004

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