Skip to main content
padlock icon - secure page this page is secure

FLT3/ ITD regulates leukaemia cell adhesion through α4β1 integrin and Pyk2 signalling

Buy Article:

$59.00 + tax (Refund Policy)

Abstract

Internal tandem duplication of FMS-like receptor tyrosine kinase 3 (FLT3/ITD) within its juxtamembrane domain is a frequent mutation in adult acute myeloid leukaemia (AML). This mutation causes constitutive activation of FLT3 and is associated with poor prognosis. The high relapse rate of FLT3/ITD-positive AML might be partly because of insufficient eradication of slow-cycling leukaemic stem cells in the bone marrow microenvironment. β1 integrin mediates haematopoietic stem and progenitor cell homing along with their retention in the bone marrow and also inhibits haematopoietic proliferation and differentiation. Here, we demonstrate that inhibition of FLT3/ITD kinase activity by a FLT3 selective inhibitor named FI-700 decreases affinity of α4β1 integrin to soluble VCAM-1. α4β1 integrin deactivation by FI-700 is independent of Rap1, which is the critical regulator of integrin inside-out signalling. In addition, selective inhibition of FLT3/ITD induces Pyk2 dephosphorylation together with the inhibition of phosphatidylinositol-3-kinase (PI3K)/Akt pathway. Both wild-type and ITD-FLT3 proteins co-immunoprecipitated with β1 integrin and Pyk2 indicating the signal crosstalk between FLT3, β1 integrin and Pyk2. These results collectively indicated that the inhibition of FLT3 kinase might contribute not only to the induction of apoptosis, but also to the leukaemia cell detachment from the bone marrow microenvironment in the treatment of AML.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: FLT3; Pyk2; leukaemia; α4β1 integrin

Document Type: Research Article

Affiliations: 1: Infectious Diseases 2: Department of Hematology and Oncology 3: Division of Cancer Biology 4: Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, Nagoya 5: Department of Biochemistry, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo, Japan

Publication date: March 1, 2011

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more