Skip to main content
padlock icon - secure page this page is secure

Fast and reliable mutation detection of the complete exon 11–15 JAK2 coding region using non-isotopic RNase cleavage assay (NIRCA)

Buy Article:

$59.00 + tax (Refund Policy)

Abstract

The screening for JAK2 V617F mutation in patients with polycythemia vera, essential thrombocythemia, and idiopathic myelofibrosis offers crucial information for the final diagnosis of these disorders. Recently, several JAK2 exons 12 and 14 mutations have been detected in V617F-negative patients with idiopathic erythrocytosis. The need for a rapid and accurate assay for the mutation screening in both exons 12 and 14 prompted us for the application of a method for the analysis of the entire coding region between exons 11 and 15. We applied the non-isotopic RNase cleavage assay and the accuracy of the method was verified in a series of V617F-positive, V617F-negative patients and healthy individuals, with no false results. This method can be applied in any laboratory without the requirement of specific sophisticated equipment.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: JAK2; NIRCA; myeloproliferative disorders

Document Type: Research Article

Affiliations: 1: First Department of Internal Medicine, Democritus University of Thrace, Alexandroupolis 2: Department of Immunology and Histocompatibility, University of Thessaly, Larissa, Greece

Publication date: September 1, 2009

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more